cooled digital scmos camera pco panda 4.2  (Excelitas corp)

Journal: bioRxiv

Article Title: Transcranial ultrasound stimulation modulates neuronal membrane potentials across broad timescales in the awake mammalian brain

doi: 10.1101/2025.06.09.658675

Figure Lengend Snippet: (a) Experimental setup: awake mice expressing SomArchon in the cortex were imaged using a custom confocal microscope, equipped with a 637 nm laser, a digital micromirror device (DMD) for patterned excitation, a high-speed sCMOS camera, and a 16× objective. A 6 mm planar transducer delivered TUS via ultrasound gel. (b) Example field of view with SomArchon-GFP neurons; Vm and spikes from circled neuron shown in (d) (scale bar: 50 μm). (c) TUS protocol: 350 kHz ultrasound, 1 s duration, 20% duty cycle, delivered at 10 or 40 Hz PRF. (d) Vm traces of example neurons (circled in (b)) during three trials of 10 Hz TUS. Red ticks: spikes; black: Vm; gray ticks: pulse onsets; shaded gray: 1 s TUS. (e) Free-field acoustic pressure in water (peak: 401 kPa). (f) Simulated in situ acoustic pressure map in mouse brain using k-Wave (peak: 209 kPa).

Article Snippet: Briefly, the widefield microscope was equipped with a Hamamatsu ORCA Fusion Digital sCMOS camera (Hamamatsu Photonics K.K., C14440-20UP), a 40x NA = 0.8 water immersion objective (Nikon, CFI APO NIR), a 140-mW 637 nm laser (Coherent Obis 637-140X) coupled with a reverse 2x beam expander (ThorLabs Inc., GBE02-E) to achieve an illumination with a diameter of 30–40 μm, and a mechanical shutter (Newport corp., model 76995) controlled laser timing via a NI DAQ board (USB-6259).

Techniques: Expressing, Microscopy, Ultrasound Gel, In Situ